Abstract
A BODIPY-based fluorescent chemosensor 1 selectively detects Zn2+ by significant fluorescence enhancement. The binding ratio of the 1-Zn2+ complex was determined from a Job plot to be 1:1. The binding constant (Ka) of Zn2+ binding to chemosensor 1 was found to be 5.9×104M−1, with a detection limit of 0.59μM. Furthermore, the 1-Zn2+ complex can specifically detect pyrophosphate (PPi) through fluorescence quenching. The detection limit for detecting PPi was found to be approximately 17.5μM. Fluorescence microscopy imaging using RAW264.7 cells showed that chemosensor 1 can be used as an effective fluorescent probe for detecting Zn2+ and PPi in living cells.
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