Zinc (Zn) mitigates copper (Cu) toxicity and retrieves yield and quality of lettuce irrigated with Cu and Zn-contaminated simulated wastewater.

Abstract

Owing to a competitive interaction, zinc (Zn) contained in highly Cu-contained wastewater was hypothesized to mitigate Cu toxicity-induced negative effects on the growth and quality of lettuce. Thus, growth, metal accumulation and biochemical responses of lettuce irrigated with simulated wastewater (SW, control), Cu-contaminated SW (CuSW, 20mg Cu L-1), Zn-contaminated SW (ZnSW, 100mg Zn L-1) and both Cu- and Zn-contaminated SW (CuZnSW, 20mg Cu and 100mg Zn L-1) were evaluated. Results revealed that irrigation with CuSW negatively affected growth (dry matters, root length and plant height) and quality (low mineral concentrations) of lettuce, which were associated with higher Cu uptake. Irrigation with Zn + Cu-contaminated SW retrieved Cu toxicity and improved root and shoot dry matters and root length by 13.5%, 46% and 19%, respectively compared to that with alone Cu-contaminated SW. Moreover, CuZnSW improved lettuce leaf quality compared to CuSW and increased concentrations of Mg (30%), P (15%), Ca (41%), Mn (24%) and Fe (23%). Moreover, compared to CuSW, CuZnSW improved flavonoids (54%), total polyphenolic compounds (1.8-fold), polyphenolic acids (77%) and antiradical activities (16.6%). Most importantly, Zn addition boosted up lettuce Cu tolerance index by 18% under Cu-contaminated SW treatment. Pearson's correlation analysis among various growth and mineral parameters demonstrated that shoot Zn concentration was positively related to elemental concentrations, phytochemical contents and antioxidant activity under Cu-contaminated environment. Thus, it is concluded that Zn supplementation retrieves negative effects of Cu toxicity to lettuce grown with Cu-contaminated wastewater.