Zinc uptake and distribution in ivy (Hedera helix L.) leaves

Jana Marešová,Martin Pipíška,Jozef Augustín,Miroslav Horník

doi:10.36547/nbc.1289

Jana Marešová, Martin Pipíška + Show 2 more

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https://doi.org/10.36547/nbc.1289

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Abstract

Detached leaves of ivy (Hedera helix L.) were used as a model for the study of zinc uptake and transport in vascular plants. By the uptake via the surface of fully immersed leaves in 25 % Hoagland nutrient media (HM) spiked with 65ZnCl2 (50 μmol/dm3 ZnCl2), concentration in leaves 4.98 μg Zn/g (dry wt.), i. e. 2.6 μg Zn/dm2 leaf area after 7d exposition were obtained. By the uptake via immersed stalks of not immersed (transpiring) leaves concentrations up to 370 μg Zn/g (dry wt.) were obtained. When Zn enters into detached leaves via the surface of immersed leaf blades, zinc is uniformly distributed in leaf blades and leaf stalks. When zinc enters detached leaves via immersed stalks of non-immersed transpiring leaves, only small part of zinc is transported to leaf blades and the prevailing part remains in leaf stalks. Stalks act as a trap, able to prevent other leaf tissues against inhibitory effects of high Zn concentrations. Mineral nutrient salts in solutions mobilize Zn trapped in leaf stalks and facilitate Zn transport by transpiration stream to leaf blades, what means that Zn in stalks is bound in ion-exchageable forms.

Highlights

Foliar uptake of mineral nutrients is of practical importance in agriculture and the problem was well described in numerous patents, periodicals and reviews (MARSCHNER, 1995)
Leaf blades of detached leaves were fully immersed in 10 ml of 25% Hoagland nutrient media (HM) supplemented with 5μmol/dm3 ZnCl2 spiked with 65ZnCl2 in plastic Petri dishes covered with lids (Fig. 1B), under the conditions described in the previous paragraph
As in the case of Zn uptake via leaf stalks (Fig. 2A), in the case of Zn uptake via leaf blade surfaces of fully immersed leaves (Fig. 2B), Zn uptake increased with increasing initial Zn concentration C0

Summary

Introduction

Foliar uptake of mineral nutrients is of practical importance in agriculture and the problem was well described in numerous patents, periodicals and reviews (MARSCHNER, 1995). Zinc is an essential nutrient as a trace element for animals, plants, and microorganisms. Studies with wheat showed good transport of Zn from stalks and leaves to developing grain (PEARSON et al, 1995; 1996), as well as from one root to another (PEARSON and RENGEL, 1995), indicating involvement of phloem transport. The movement of foliar - applied Zn to plant roots was demonstrated in small number of studies (HASLETT et al, 2001).

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