Zinc is an inhibitor of the stimulatory response of the sodium efflux to the microinjection of cyclic AMP and forskolin in single barnacle muscle fibers

Abstract

The main aim of the experiments with which this paper deals was to test the hypothesis that Zn is an inhibitor of the stimulatory response of the ouabain-insensitive component of the Na efflux to the injection of cAMP, DcAMP (dibutyryl-cAMP) and FD (forskolin derivative) in barnacle muscle fibers. The results obtained were as follows: (1) External application of Zn caused a fall in the Na efflux in fibers poisoned with ouabain beforehand; (2) external application of Zn prior to the injection of cAMP, DcAMP and FD led to a marked reduction in the response of the Na efflux in fibers pre-treated with ouabain; (3) the response obtained by the injection of DcAMP and FD into ouabain-poisoned fibers pre-exposed to Zn was small but sustained; and (4) external application of Zn following peak stimulation by injecting DcAMP or FD led to reversal of this response. (Parallel experiments involving the injection of cAMP were not done, since the response following the onset of peak stimulation decays quite rapidly.) Taken together, these results support the hypothesis that Zn behaves as an inhibitor of the stimulatory response obtained by the injection of cAMP, DcAMP and FD.