Abstract
Chagas disease (CD), caused by the flagellate protozoan Trypanosoma cruzi, is one of the major public health problems in developing countries. Benznidazole (BNZ) is the only drug available for CD treatment in most countries, however, it presents high toxicity and low bioavailability. To address these problems this study used Zeolitic Imidazolate Framework-8 (ZIF-8), which has garnered considerable attention due to its potential applications, enabling the controlled delivery of drugs. The present work developed and characterized a BNZ@ZIF-8 system, and the modulation of BNZ release from the ZIF-8 framework was evaluated through the in vitro dialysis release method under sink conditions at different pH values. Moreover, the in vitro evaluation of cell viability and cytotoxicity by MTT assay were also performed. The dissolution studies corroborated that a pH sensitive Drug Delivery System capable of vectorizing the release of BNZ was developed, may leading to the improvement in the bioavailability of BNZ. The MTT assay showed that no statistically significant toxic effects occurred in the developed system, nor significant effects on cell viability.
Highlights
Chagas disease (CD), caused by the flagellate protozoan Trypanosoma cruzi, is one of the major public health problems in developing countries
This study aimed to analyze the release kinetics and cell viability of a new drug delivery system based on BNZ carried by the Zeolitic Imidazolate Framework-8 (ZIF-8) framework, using in vitro dialysis and cytotoxicity tests in order to provide the necessary subsidies for the development of new pharmaceutical products for Chagas disease
Based on the scan (200–1000 nm) (Fig. 1), the BNZ solution (15 μg mL−1) had maximum absorption at 323 nm, while the Zeolitic Imidazolate Framework (ZIF)-8 at 243 nm
Summary
Chagas disease (CD), caused by the flagellate protozoan Trypanosoma cruzi, is one of the major public health problems in developing countries. Benznidazole (BNZ) is the only drug available for CD treatment in most countries, it presents high toxicity and low bioavailability. To address these problems this study used Zeolitic Imidazolate Framework-8 (ZIF-8), which has garnered considerable attention due to its potential applications, enabling the controlled delivery of drugs. Chagas disease or American trypanosomiasis is a neglected tropical disease caused by the flagellated protozoan Trypanosoma cruzi It is endemic in about 21 developing countries in Latin America, it is a public health problem on other continents due to the intense immigration movement of the infected population. Efforts are being made to develop more effective dosage forms, capable of increasing patient compliance and reduced toxic effects[4,5,6]
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