Zeta potential of anoxygenic phototrophic bacteria and Ca adsorption at the cell surface: Possible implications for cell protection from CaCO3 precipitation in alkaline solutions

Abstract

Electrophoretic mobility measurements and surface adsorption of Ca on living, inactivated, and heat-killed haloalkaliphilic Rhodovulum steppense, A-20s, and halophilic Rhodovulum sp., S-17-65 anoxygenic phototrophic bacteria (APB) cell surfaces were performed to determine the degree to which these bacteria metabolically control their surface potential equilibria. Zeta potential of both species was measured as a function of pH and ionic strength, calcium and bicarbonate concentrations. For both live APB in 0.1M NaCl, the zeta potential is close to zero at pH from 2.5 to 3 and decreases to −30 to −40mV at pH of 5–8. In alkaline solutions, there is an unusual increase of zeta potential with a maximum value of −10 to −20mV at a pH of 9–10.5. This increase of zeta potential in alkaline solutions is reduced by the presence of NaHCO3 (up to 10mM) and only slightly affected by the addition of equivalent amount of Ca. At the same time, for inactivated (exposure to NaN3, a metabolic inhibitor) and heat-killed bacteria cells, the zeta potential was found to be stable (−30 to −60mV, depending upon the ionic strength) between pH 5 and 11 without any increase in alkaline solutions. Adsorption of Ca ions on A-20s cells surface was more significant than that on S-17-65 cells and started at more acidic pHs, consistent with zeta potential measurements in the presence of 0.001–0.01mol/L CaCl2. Overall, these results indicate that APB can metabolically control their surface potential to electrostatically attract nutrients at alkaline pH, while rejecting/avoiding Ca ions to prevent CaCO3 precipitation in the vicinity of cell surface and thus, cell incrustation.