Abstract
Newly fertilized zebrafish (Danio rerio) embryos were exposed to increasing concentrations of the selective serotonin reuptake inhibitor (SSRI) citalopram from fertilization until six days post-fertilization (dpf). Locomotor activity data were acquired at six dpf using an automated ZebraBox® infrared tracking system. Individual (n = 32) locomotor activity was recorded during 75 minutes in total during alternating illumination conditions (0% light, i.e. dark periods, and 100% light, i.e. light periods). The first 15 minutes of the test consisted of a dark period, i.e an acclimatization phase. Afterward, six alternating light and dark periods were conducted. Individual zebrafish embryo-larvae locomotion was tracked and aggregated in ten-second bins. The dataset, containing nine locomotor-related quantified endpoints (factors), was parsed, analyzed, and visualized using R software. The dataset and its associated custom R script may be used to further explore locomotor activity outcomes (e.g. anxiolytic or anxiogenic properties) following exposure to citalopram or other neuroactive chemicals.
Highlights
Zebrafish (Danio rerio) embryo-larvae locomotor activity data analysis: Evaluating anxiolytic effects of the antidepressant compound citalopram
Fertilized zebrafish (Danio rerio) embryos were exposed to increasing concentrations of the selective serotonin reuptake inhibitor (SSRI) citalopram from fertilization until six days postfertilization
Individual (n 1⁄4 32) locomotor activity was recorded during 75 minutes in total during alternating illumination conditions (0% light, i.e. dark periods, and 100% light, i.e. light periods)
Summary
Biology Behavioral Neuroscience, Developmental Neuroscience, Developmental Neurotoxicology, Developmental Toxicology, Aquatic toxicology, Environmental Toxicology, Pharmacology, Toxicology Figure Infrared tracking using ZebraBox® apparatus with Zebralab software (Viewpoint, Lyon, France) Comma-separated value (.csv) data files Zebrafish embryo-larvae displaying obvious malformations (e.g. non-inflated swim bladder) or other visible effects (e.g. mortality) were omitted from data analysis Fertilized zebrafish embryos were placed individually in 96-well plates together with 200 mL test solution. Value of the Data Fish embryo locomotor activity is a sensitive measurement of anxiogenic and/or anxiolytic properties of chemical compounds, in the present case citalopram. The data and associated R scripts are made available to researchers who would like to reproduce analysis of high-throughput fish embryo-larvae locomotor activity datasets.
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