Abstract
Zearalenone (ZEN) is a mycotoxin produced by members of the genus Fusarium. ZEN detection by ELISA using single chain fragment variable (scFv) represents a novel means of mycotoxin detection and diagnosis of fungal infection. To develop immunoassay of ZEN based on scFv, ZEN was conjugated to horseradish peroxidase (HRP). ELISA and SDS-PAGE analysis showed that the covalent attachment of ZEN to HRP was successful. Anti-ZEN scFv-E-tag fusion protein was used to detect ZEN by competitive direct-ELISA (CD-ELISA) method, and the results indicated that this scFv was appropriate in ZEN detection, meaning that scFv against fungal toxin could have an application in mycotoxin detection and diagnosis of fungal infection.
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