Abstract
Summary Soil-borne wheat mosaic virus (SBWMV) was detected in Poland for the first time in 2010 in the Southern Greater Poland and in the following years in the Lower Silesia and in the Pomerania regions. All found isolates induced mild symptoms of disease. Stunting without typical leaf mosaic of infected plants was observed. A low concentration of the virus in plant sap caused troubles in diagnostics. Difficulties in interpreting of obtained ambiguous results of ELISA test and RT-PCR technique were the main problem. The specific primers and real-time RT-PCR conditions were developed to improve sensitivity and of this assay for the detection of SBWMV. It can be used when the virus concentration in infected plants is very low and the standard ELISA test and RT-PCR technique failed.
Highlights
The specific primers and real-time RT-PCR conditions were developed to improve sensitivity and of this assay for the detection of Soil-borne wheat mosaic virus (SBWMV)
Opublikowane dane (Ratti i wsp. 2004) wskazują, że technika real-time RT-PCR jest dziesięć razy czulsza i szybsza od standardowej techniki RT-PCR, ponieważ nie wymaga elektroforetycznego rozdziału powstałych produktów reakcji w żelu agarozowym ani sekwencjonowania w celu sprawdzenia specyficzności uzyskanej sekwencji nukleotydów
2. Opracowana technika real-time RT-PCR może być wykorzystywana w rutynowej diagnostyce, a także do weryfikacji niejednoznacznych wyników testu DASELISA i techniki RT-PCR
Summary
The specific primers and real-time RT-PCR conditions were developed to improve sensitivity and of this assay for the detection of SBWMV. It can be used when the virus concentration in infected plants is very low and the standard ELISA test and RT-PCR technique failed. Streszczenie Wirus odglebowej mozaiki pszenicy (Soil-borne wheat mosaic virus, SBWMV) wykryto w Polsce po raz pierwszy w 2010 roku, w południowej Wielkopolsce, a w kolejnych latach na Dolnym Śląsku i na Pomorzu.
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