Abstract
BackgroundProstate cancer (PCa) is one of the most common malignancies in men. YTHDF1 may play an important role in promoting PCa progression, but there is no reports to date on YTHDF1 function in PCa.ObjectiveThis study explored whether YTHDF1 could regulate TRIM44 in PCa cells.MethodsBy querying the TCGA database, we evaluated YTHDF1 expression in PCa, the OS and DFS of YTHDF1, and the correlation between YTHDF1 and TRIM44 in PCa. We constructed vectors to interfere with YTHDF1 expression and overexpress TRIM44 to examine the role of YTHDF1 and TRIM44 in PCa cells. Differentially expressed mRNAs were identified by mRNA sequencing. The levels of YTHDF1, TRIM44, LGR4, SGTA, DDX20, and FZD8 were measured by qRT-PCR and WB was used to determine YTHDF1 and TRIM44 expression. A CCK-8 assay was used to assess cell proliferation. A Transwell chamber assay was used measure cell migration and invasion ability.ResultsYTHDF1 was highly expressed in both Pca tissues and cells. PCa patient prognosis with high YTHDF1 expression was relatively poor. Cell function experiments showed that inhibiting YTHDF1 expression decreased cell proliferation, migration, and invasion. RNA sequencing analysis revealed that YTHDF1 may promote PCa cell proliferation, migration, and invasion by modulating TRIM44 expression. Cell function experiments further verified that YTHDF1 promoted PCa cell proliferation, migration, and invasion by regulating TRIM44.ConclusionsYTHDF1 enhances PCa cell proliferation, migration, and invasion by regulating TRIM44.
Highlights
Prostate cancer (PCa) is the most common cancer of solid organs in men and the second most common cause of cancerrelated deaths in men (Nguyen-Nielsen and Borre 2016)
To detect YTHDF1 expression in PCa, we compared 492 PCa tissues (T) and 152 paracancerous tissues (N) by querying the TCGA database and found that YTHDF1 was highly expressed in PCa (P = 0.003, Fig. 1A)
The results indicate that PCa patients with high YTHDF1 expression have a relatively poor prognosis
Summary
Prostate cancer (PCa) is the most common cancer of solid organs in men and the second most common cause of cancerrelated deaths in men (Nguyen-Nielsen and Borre 2016). M6A plays an important role in PCa and YTHDF2 is involved in regulating PCa progression (Li et al 2018). The prognosis and survival rate of PCa patients with high expression is lower, suggesting that YTHDF1 may play a role in promoting PCa progression. YTHDF1 may play an important role in promoting PCa progression, but there is no reports to date on YTHDF1 function in PCa. Objective This study explored whether YTHDF1 could regulate TRIM44 in PCa cells. Cell function experiments showed that inhibiting YTHDF1 expression decreased cell proliferation, migration, and invasion. RNA sequencing analysis revealed that YTHDF1 may promote PCa cell proliferation, migration, and invasion by modulating TRIM44 expression. Cell function experiments further verified that YTHDF1 promoted PCa cell proliferation, migration, and invasion by regulating TRIM44. Conclusions YTHDF1 enhances PCa cell proliferation, migration, and invasion by regulating TRIM44
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