YourTuber matters: Screening for potato variety for the synthesis of bacterial cellulose in its tuber juice

Abstract

This study aimed to characterize potato varieties for producing potato juice media (PJM) that allow bacterial cellulose (BC) effective and cost-efficient production. The study used 12 edible and 10 starch potato varieties from an accredited company for breeding and seed production. In general, edible varieties produced a 73 % higher PJ yield. Favorable BC yields were obtained using five edible and two starch varieties. Notably, the average BC yields in PJM from three edible varieties (Altesse, Mazur, and Owacja) were above the average BC yield from Hestrin-Schramm (HS) medium (4.3, 4.1, and 3.9 g/L v. 3.69 g/L, respectively); these varieties had relatively high concentrations of glucose (3.3–4.2 g/L), fructose (3.0–4.2 g/L), and sucrose (2.9–4.2 g/L). It was also shown that the macro- and microstructure, crystallinity, and polymerization degree showed no significant differences between PJM-derived BC and HS-BC. As estimated, the cost of PJM required to produce 1 kg of BC is approximately EUR 60. In contrast, the cost of HS medium exceeds 1200 EUR. In conclusion, our research has proven that PJM can significantly reduce the costs (by over tenfold) of the medium for BC biosynthesis, ultimately lowering overall costs of producing this valuable biomaterial.