Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Mice.

Tiange Li,Yue Zhang,Min Du,Xueying Mao,Jiajia Song,Lijun Chen

doi:10.3390/nu14020279

Abstract

The effects of synbiotic yogurt supplemented with inulin on the pathological manifestations and gut microbiota–bile acid axis were investigated using a dehydroepiandrosterone (DHEA)-induced polycystic ovary syndrome (PCOS) mice model. Female C57BL/6J mice were injected subcutaneously with DHEA at a dose of 6 mg/100 g BW for 20 days to establish a PCOS mouse model. Then, the PCOS mice were treated with yogurt containing inulin (6% w/w) at 15 mL/kg BW for 24 days. Results showed that supplementation of synbiotic yogurt enriched with inulin to PCOS mice decreased the body weight gain, improved estrus cycles and ovary morphology, and reduced the levels of luteinizing hormone while increasing the levels of follicle-stimulating hormone and interleukin-22 in serum. At the genus level, synbiotic yogurt increased the relative abundance of Lactobacillus, Bifidobacterium, and Akkermansia. PICRUSt analysis indicated that KEGG pathways including bile acid biosynthesis were changed after inulin-enriched synbiotic yogurt supplementation. Synbiotic yogurt enriched with inulin also modulated the bile acid profiles. In conclusion, inulin-enriched synbiotic yogurt alleviated reproductive dysfunction and modulated gut microbiota and bile acid profiles in PCOS mice.

Highlights

Polycystic ovary syndrome (PCOS) is a common heterogeneous endocrine disease with a high prevalence in reproductive-aged women worldwide [1]. This disorder is the primary cause of female infertility defined by a combination of ovarian dysfunction, dysfunctional follicular maturation, and ovarian hormone dysregulation, manifesting as hyperandrogenism and the hypersecretion of luteinizing hormone (LH) [2]
The serum hormones associated with ovarian function including E2, T, LH, follicle-stimulating hormone (FSH), prolactin PRL, and PROG were measured
Our study indicated a significant reduction in IL-6 and TNF-α in PCOS mice after synbiotic yogurt treatment

Summary

Introduction

Polycystic ovary syndrome (PCOS) is a common heterogeneous endocrine disease with a high prevalence in reproductive-aged women worldwide [1]. This disorder is the primary cause of female infertility defined by a combination of ovarian dysfunction, dysfunctional follicular maturation, and ovarian hormone dysregulation, manifesting as hyperandrogenism and the hypersecretion of luteinizing hormone (LH) [2]. Women suffering from PCOS have an elevated risk of obesity, insulin resistance, hypertension, cardiovascular disease, and other types of metabolic dysfunction [4]. Due to the high prevalence and association with multiple metabolic diseases, controlling the development of PCOS is of great importance for the reproductive health of women. Recent studies showed that the dysbiosis of gut microbiota is linked to the progression of PCOS [6]

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Conclusion