Abstract
目的 探讨拉米夫定治疗1年后慢性乙型肝炎(CHB)患者发生YMDD基序变异的状况及其检测方法.方法 采用荧光定量PCR法检测血清HBV DNA含量,荧光PCR法和变性高效液相色谱法检测HBV YMDD基序变异.结果 61例CHB患者经过拉米夫定治疗1年后,40例(65.6%)HBV DNA阴转;在21例HBV DNA阳性患者中,10例为YMDD野生型,11例出现YMDD基序变异,变异率达18.0%(11/61).在11例基序变异中,完全变异7例(63.6%),部分变异4例(36.4%).在完全变异型中,YIDD变异型5例,YVDD变异型2例;在部分变异型中,YIDD变异型3例,YVDD变异型1例.结论 CHB患者拉米夫定治疗1年后,出现较高的YMDD基序变异率.利用荧光PCR法结合变性高效液相色谱法检测基序变异,经济便捷,可作为CHB患者YMDD基序变异的常规监测。
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