Abstract
The objective of this work was to evaluate the yield performance of two generations (BC2F2 and BC2F9) of introgression lines developed from the interspecific cross between Oryza sativa and O. glumaepatula, and to identify the SSR markers associated to yield. The wild accession RS‑16 (O. glumaepatula) was used as donor parent in the backcross with the high yielding cultivar Cica‑8 (O. sativa). A set of 114 BC2F1 introgression lines was genotyped with 141 polymorphic SSR loci distributed across the whole rice genome. Molecular analysis showed that in average 22% of the O. glumaepatula genome was introgressed into BC2F1 generation. Nine BC2F9 introgression lines had a significantly higher yield than the genitor Cica‑8, thus showing a positive genome interaction among cultivated rice and the wild O. glumaepatula. Seven QTL were identified in the overall BC2F2, with one marker interval (4879‑EST20) of great effect on yield. The alleles with positive effect on yield came from the cultivated parent Cica‑8.
Highlights
Due to domestication, cultivated rice (Oryza sativa L.) experienced a severe bottleneck effect that has decreased its genetic variability (Tanksley & McCouch, 1997), resulting in vulnerability to plagues and diseases and decreasing its ability of adapting to adverse field conditions
The objective of this study was to evaluate the yield performance of two generations (BC2F2 and BC2F9) of introgression lines developed from the interspecific cross between O. sativa x O. glumaepatula, and to identify SSR markers associated to yield
These plants were individually self‐fertilized without selection until the F9 generation, using the single seed descent (SSD) method, which resulted in 114 BC2F9 introgression lines (IL)
Summary
Due to domestication, cultivated rice (Oryza sativa L.) experienced a severe bottleneck effect that has decreased its genetic variability (Tanksley & McCouch, 1997), resulting in vulnerability to plagues and diseases and decreasing its ability of adapting to adverse field conditions. Variations of the advanced backcross‐QTL (AB‐QTL) strategy, proposed by Tanksley & Nelson (1996), have been implemented to assist the introgression of wild chromosome segments in the genetic backgrounds of elite rice cultivars (Rangel et al, 2005; McCouch et al, 2007; Cheema et al, 2008) This strategy allows the identification of alleles related with QTL. Yield QTL analysis of Oryza sativa x O. glumaepatula positive effects and the development of introgression lines (IL) These IL can be better described as a set of near‐isogenic lines (NIL) developed through a series of backcrosses, containing wild segments in a uniform, cultivated genetic background (Tian et al, 2006; Barone et al, 2009). These lines can be maintained by self‐pollination and can be very effective in QTL identification and validation because any phenotypic difference between an IL and the recurrent parent can be attributed solely to the donor alleles in the introgressed segment (Lippman et al, 2007)
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