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Abstract
In the preceding paper in this series it was shown that Type A Clostridium parabotulinum when grown under accurately controlled conditions on a casein digest – glucose – corn steep medium produced large and consistent yields of toxin. In this paper a modified procedure is described. Cultures were inoculated in the usual manner and incubated for 10 hr., or until growth reached the upper third of logarithmic increase. Concentrate of the same medium was then added by a continuous drip method for 18 to 60 hr., or until the concentration of the medium was doubled to quadrupled.Under these circumstances the organisms continued to increase for some 40 hr. while in the controls in unconcentrated media growth ceased at 18 to 20 hr. The yield of toxin was increased two to three times over the controls in unconcentrated media.
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