Yersinia massiliensis (Enterobacteriales: Enterobacteriaceae) in the host Anaphes nitens (Hymenoptera: Mymaridae): first report of association with insects.

M F Ribeiro,M A Soares,A L Favoreto,A J V Zanuncio,B R De Marchi,C F Wilcken,J C Zanuncio,C Jordan,V R Carvalho

doi:10.1590/1519-6984.237098

Abstract

Endosymbiont bacteria can affect biological parameters and reduce the effectiveness of natural enemies in controlling the target insect. The objective of this work was to identify endosymbiont bacteria in Anaphes nitens (Girault, 1928) (Hymenoptera: Mymaridae), the main natural enemy used to manage Gonipterus platensis (Marelli, 1926) (Coleoptera: Curculionidae). Genomic DNA from six A. nitens populations was extracted and polymerase chain reactions (PCR) were performed with the primers to detect endosymbiont bacteria in this insect. The PCR products were amplified, sequenced, and compared with sequences deposited in the GenBank for the bacteria identification. All A. nitens populations had the bacterium Yersinia massiliensis (Enterobacteriales: Enterobacteriaceae). This bacterium was originally described as free-living, and it is associated with and composes part of the A. nitens microbiota. This is the first report of Y. massiliensis in an insect host.

Highlights

The use of the egg parasitoid Anaphes nitens (Girault, 1928) (Hymenoptera: Mymaridae) is the main management strategy for the Eucalyptus spp. defoliator beetle, Gonipterus platensis (Marelli, 1926) (Coleoptera: Curculionidae) (Souza et al, 2016)
BLASTN analysis of the 600 bp amplicon resulted in high identity (98% identity with the accession number CP028487-1) with the bacterium Yersinia massiliensis (Enterobacteriales: Enterobacteriaceae)
This bacterium was found in all A. nitens populations and it was absent in G. platensis eggs and excrement layer of the egg capsules (Figure 1)

Summary

Introduction

The use of the egg parasitoid Anaphes nitens (Girault, 1928) (Hymenoptera: Mymaridae) is the main management strategy for the Eucalyptus spp. defoliator beetle, Gonipterus platensis (Marelli, 1926) (Coleoptera: Curculionidae) (Souza et al, 2016) This parasitoid is originally from Australia (Mapondera et al, 2012), and it has been successfully introduced in South Africa, Spain and California (United States of America) (Cordero-Rivera et al, 1999; Hanks et al, 2000) but with low efficiency in some regions of Portugal, Chile and Brazil (Reis et al, 2012; Gumovsky et al, 2015; Souza et al, 2016). There are no reports of endosymbiont bacteria in A. nitens

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Conclusion