Yeni 10 STR Lokusunun Türkiye'deki Gen Sıklığının Belirlenmesi: Deneysel Çalışma

Abstract

Objective: Short tandem repeats (STRs) have been widely used in human identification in forensics. Evaluation and design of new markers are useful tools to obtain additional information and complete conventional analysis. Also, it will be an alternative way to confirm the results in the problematic cases (complex kinship, degraded samples, etc.). Increasing the number of loci will result in reducing the risk of adventitious matches in countries that have a national DNA database. In this study, we investigated allele frequencies of the 10 non-Combined DNA Index System STR loci (D7S1517, D3S1744, D12S391, D2S1360, D6S474, D4S2366, D8S1132, D5S2500, D21S2055, D10S2325) by using Investigator® HDplex kit. Material and Methods: DNAs were extracted from 100 blood samples by using the QIAmp DNA Mini Kit (Qiagen). STR loci were amplified according to Investigator® HDplex kit. Polymerase chain reaction products were separated with ABI 3130 Genetic Analyzer and analyzed with GeneMapper IDX software. Forensic and population parameters were estimated with the Promega PowerStats Excel sheet. Allele frequencies, p-values for Hardy-Weinberg equilibrium, and population differentiation based on the loci (Fst) were calculated with Arlequin ver.3.5. Results: We observed that all STR loci showed high power of discrimination. For population comparison, we found statistically significant differences between Türkiye and African, East Asian, and American populations at a couple of STR loci. Besides, there was no statistically significant variation between Turkish and European populations as expected. Conclusion: This study provides Turkish population data for forensic laboratories and this kit can be used to support the existing STR loci.