Yellow perch strain evaluation I: Genetic variance of six broodstock populations

Abstract

As a prelude to strain selection for domestication and future marker assisted selection, genetic variation revealed by microsatellite DNA was evaluated in yellow perch, Perca flavescens, from four wild North American populations collected in 2003–2004 (Maine, New York, North Carolina, and Pennsylvania,), and two captive populations (Michigan and Ohio). For the loci examined, levels of heterozygosity ranged from H e = 0.04 to 0.88, genetic differentiation was highly significant among all population pairs, and effective migration ranged from low ( N e m = 0.3) to high ( N e m = 4.5). Deviation from Hardy–Weinberg equilibrium was regularly observed indicating significant departures from random mating. Instantaneous measures of inbreeding within these populations ranged from near zero to moderate (median F = 0.16) and overall inbreeding levels averaged F IS = 0.18. Estimates of genetic diversity, Φ ST, and genetic distance were highest between Michigan and all other broodstock groups and lowest between New York and Ohio. Genetic differentiation among groups did not correlate with geographic distance. Overall, the patterns of variation exhibited by the captive (Michigan and Ohio) populations were similar to patterns exhibited by the other wild populations, indicating that spawning and management practices to date have not significantly reduced levels of genetic variation.