Yellow mombin and jackfruit seeds residues applied in the production of reducing sugars by a crude multi‐enzymatic extract produced by Penicillium roqueforti ATCC 101110

Abstract

As an alternative to the use of widely investigated agro-industrial residues, the present study aimed to promote the valorization of two selected residues, yellow mombin seed (YS) and jackfruit seed (JS), as a result of their enhanced performance. YS was applied as a solid state substrate for Penicillium roqueforti ATCC 101110 cultivation (25 °C, Aw = 0.963, 107 spores g-1 and 142 h) to produce a crude multi-enzymatic extract (CE-YS) containing activities of CMCase = 31.95 U g-1 , xylanase = 56.85 U g-1 , exoglucanase = 5.55 U g-1 and FPase = 24.60 U g-1 . CE-YS was then applied to six different residues saccharification and the best performance was obtained with jackfruit seed residue (JS), which was selected for enzymatic saccharification. The highest productivity of reducing sugars expressed as glucose (6.26 mg g-1 h-1 ) was obtained under the conditions: 40.7 g L-1 JS, 5 mmol L-1 MgCl2 , 65 °C, 120 rpm, pH 3.0 (citrate buffer 50 mmol L-1 ) and 18 h. The residues, YS and JS, can be used satisfactorily for the production of bioproducts of great industrial applicability, such as crude extracts (containing cellulolytic enzymes) and RS (which can be converted, for example, into bioethanol). © 2020 Society of Chemical Industry.