Abstract
Yellow and blue forms of laccase from solid-state and submerged cultures of Panus tigrinus were isolated. Both laccases had similar molecular masses and specific activity, but yellow laccase had no `blue' maximum in the absorption spectrum. Blue laccase oxidized veratryl alcohol and a non-phenolic dimeric lignin model compound only in the presence of 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) as electron-transfer mediator. Yellow laccase catalyzed these reactions without any additional compounds. It is supposed that yellow laccase is formed as a result of blue laccase modification by products of lignin degradation. These compounds might play a role of natural electron-transfer mediators for the oxidation of non-phenolic substances, catalyzed by yellow laccase.
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