Abstract
The absence of urban yellow fever virus (YFV) in Bolivian cities has been attributed to the lack of competent urban mosquito vectors. Experiments with Aedes aegypti from Santa Cruz, Bolivia, demonstrated infection (100%), dissemination (20%), and transmission of a Bolivian YFV strain (CENETROP-322).
Highlights
The absence of urban yellow fever virus (YFV) in Bolivian cities has been attributed to the lack of competent urban mosquito vectors
An Ae. aegypti eradication campaign initiated by the Pan American Sanitary Bureau in 1947 eliminated this species from most of Central and South America, and urban YF disappeared from the Americas in the 1940s
hemagglutination inhibition (HI) results indicated that antibodies against YFV (320 titer, Table 2) developed in one mouse exposed to SC mosquitoes infected with CENETROP-322, which indicated transmission by the Bolivian Ae. aegypti
Summary
John-Paul Mutebi,* Alberto Gianella,† Amelia Travassos da Rosa,‡ Robert B. Experiments with Aedes aegypti from Santa Cruz, Bolivia, demonstrated infection (100%), dissemination (20%), and transmission of a Bolivian YFV strain (CENETROP-322). All three strains of YFV caused viremia in hamsters on day 3 after infection, with titers of 8.5, 8.7, and 7.3 TCID50log10/mL (CENETROP-322, Jimenez, and Asibi, respectively), as determined by assay in mosquito cell cultures (4). Infection rates and mean viral titers for CENETROP-322 were higher in SC Ae. aegypti than in the REX-D strain (Table 1). Virus titers in the mosquitoes varied considerably but were lowest in REX-D strain insects infected with CENETROP-322 (Table 1). HI results indicated that antibodies against YFV (320 titer, Table 2) developed in one mouse exposed to SC mosquitoes infected with CENETROP-322, which indicated transmission by the Bolivian Ae. aegypti. Transmission was confirmed by recovering YFV by culture from dead mice
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