Yeasts Isolated from Long Island Sound

Abstract

Water samples were obtained from a total of 34 stations in Long Island Sound and the Housatonic River Estuary. With the exception of the latter location, surface and mid-depth collections were made at each station. Sampling areas were chosen to permit the examination of water from a variety of depths and locations. Collections were made with an 8-liter PVC sampler (Logan Smith Associates, Harbor City, California). Fifty and 100 ml amounts of water were Millipore filtered (0.45 /) on board ship. Prior experience indicated that these quantities were suitable for detection of yeasts in Long Island Sound water. The filters were aseptically transferred to agar plates containing the isolation medium of Fell and Van Uden (5) modified to contain 500 ppm chloramphenicol to retard bacterial growth. The plates were incubated at 18 C to prevent overgrowth by filamentous fungi. In processing samples from areas of large mold populations (the Housatonic River Estuary) Wickerham's isolation technique using a rotary shaker was employed (10) in addition to the procedure described above. The membrane filters were placed in 250 ml Erlenmeyer flasks each containing 50 ml of the isolation medium in broth form. The