Yeasts Associated with the Small-Intestinal Contents and Epithelium of Pon Yang Kham (Charolais Crossbred) Fattening Beef Cattle.

Jirameth Angchuan,Nantana Srisuk,Savitree Limtong,Kannika Wongpanit,Pannida Khunnamwong

doi:10.3390/microorganisms9071444

Abstract

Yeast diversity in the pia and small-intestinal epithelium of Pon Yang Kham fattening cattle in Thailand was studied using a culture-dependent method. A total of 701 yeasts were isolated from the pia of the duodenum, jejunum, and ileum of the small intestine, while 425 isolates were obtained from the epithelium of all three parts of the small intestine. Yeast identification was performed and ascomycetous yeasts were found at levels of 96.9% and 86.8% in the pia and small intestine, respectively, whereas basidiomycetous yeasts were found at levels of 2.3% and 12.7%. Candida parapsilosis was the species with the highest occurrence in the duodenal and jejunal pia, with an 83.3% and 77.8% frequency of occurrence (FO), respectively. Both C. parapsilosis and C. tropicalis were species with the highest occurrence in the ileum, with a 61.1% FO. Moreover, C. parapsilosis was the species with the highest occurrence in the epithelium of the duodenum, jejunum, and ileum, with FOs of 88.2%, 87.5%, and 87.2%, respectively. Principal coordinate analysis revealed no marked differences in yeast communities from either the pia or epithelium of all three parts of the small intestine. An estimation of the expected richness of the species showed that the observed species richness was lower than the predicted richness.

Highlights

Pon Yang Kam fattening beef cattle are Charolais crossbred cattle and have improved the quality of beef in response to consumer demand by the Pon Yang Kham Livestock cooperatives in Sakon Nakhon Province, Thailand
Fifty-four pia samples, from 18 duodena, 18 jejuna, 18 ilea and 50 small-intestinal epithelial tissues from 17 duodena, jejuna and ilea were collected from the slaughterhouse of Pon Yang Kham Livestock Breeding Cooperative N.S.C., Ltd., in Sakon Nakhon
The results indicated that the common yeast species obtained from both pia and the epithelium of all three parts of the small intestine belonged to Ascomycota

Summary

Introduction

Pon Yang Kam fattening beef cattle are Charolais crossbred cattle and have improved the quality of beef in response to consumer demand by the Pon Yang Kham Livestock cooperatives in Sakon Nakhon Province, Thailand. These cattle were fed natural feed and crude protein comprised of casava, rice bran, palm kernel meal, molasses and urea, which were provided by the cooperative. They were not treated with hormones, antibiotics, imported feed ingredients or artificial vitamins [1]. Pia is popularly used as a cooking ingredient in many traditional dishes from Northeast Thailand because of its unique taste

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