Abstract
Since its introduction by Fields and Song (1), the two-hybrid system has been widely used to identify and explore interactions between proteins. The extreme popularity of this method has led to numerous modifications. These include many new plasmids and strains for use in the traditional yeast transcription-based assay and the adaptation of the two-hybrid system to other organisms (2–4) and to protein-nucleic acid interactions (one- and three-hybrid systems) (5–7), as well as the development of assays that do not depend on transcription (8,9). These latter modifications are described in detail in Chapters 15 – 20 . This chapter focuses on the many options available for performing a traditional yeast transcription-based two-hybrid experiment.
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