Abstract
▼The two-hybrid system (THS), first introduced by Fieldsand Song (1989) (Ref. 1), is a powerful technique for identi-fyingnew proteinsinvolved in specific biologicalprocesses.It allows for the rapid isolation of the gene that codes fora protein that interacts with a specific protein of interest.Here, we present not a review of the current THS technol-ogy but, rather, a comprehensive guide designed to takethe reader through a THS screen. Readers not familiar withthe THS are asked to review (Ref. 1, 2, 3, 4, 5). Currently,there are a number of different versions of the THS avail-able. This article describes procedures useful for the ‘Fields’THS, based on the
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