Abstract
ADP and Ap 3A are synthesized by the yeast phenylalanyl-tRNA synthetase, according to reaction pathways similar to the pyrophosphorolysis of the intermediate aminoacyladenylate or to the one leading to Ap 4A synthesis. The enzyme-bound phenylalanydenylate reacts with inorganic phosphate or ADP to yield, respectively, ADP or Ap 3A. The rate of synthesis is strongly stimulated by Zn 2+. This new phosphorolysis activity accounts for the complex pattern of bisnucleoside polyphosphate syntheses starting from ATP.
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