Abstract
Cell processing occurs in many technologies such as lab-on-a-chip, biopharmaceutical manufacturing, and food and beverage industry. Centrifuges and filters are used in preprocessing and filtration stages. These technologies are not continuous flow filtration methods, a drawback for automation and miniaturization. Continuous cell filtration using ultrasonic standing waves has been successfully used at limited flow rates [Hawkes and Coakley, Enzyme Microbial Technol. 19, 57–62 (1996)]. Advantages of ultrasonic particle filtration are continuous operation with no mechanical moving parts, no risk of membrane fouling, and no consumables. We present a novel design of an acoustophoretic particle separation system operating at large volume flow rates. The technology operates by creating ultrasonic standing waves that produce an acoustic radiation force on particles which exceeds the drag and gravitational forces thereby trapping the particles. Over time aggregation of trapped particles results in gravitational settling of the agglomerated particles. The system comprises a 1 in. × 1 in. flow section and is powered by a 2 MHz PZT-8 transducer and typically operates at flow rates up to 2 L/H. Concentration reductions in excess of 90% are obtained for yeast suspensions of rehydrated S. cerevisiae in RO-DI water with volume concentrations ranging from 0.5 to 3%. [Work supported by NSF PFI:BIC 1237723.]
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
