Abstract
One of the most effective strategies to enhance metabolite biosynthesis and accumulation in biotechnological systems is the use of elicitation processes. This study assesses the influence of different concentrations of yeast extract (YE) on ginsenoside biosynthesis in Panax quinquefolium (American ginseng) hairy roots cultivated in shake flasks and in a nutrient sprinkle bioreactor after 3 and 7 days of elicitation. The saponin content was determined using HPLC. The maximum yield (20 mg g−1 d.w.) of the sum of six examined ginsenosides (Rb1, Rb2, Rc, Rd, Re and Rg1) in hairy roots cultivated in shake flasks was achieved after application of YE at 50 mg L−1 concentration and 3 day exposure time. The ginsenoside level was 1.57 times higher than that attained in control medium. The same conditions of elicitation (3 day time of exposure and 50 mg L−1 of YE) also favourably influenced the biosynthesis of studied saponins in bioreactor cultures. The total ginsenoside content was 32.25 mg g−1 d.w. and was higher than that achieved in control medium and in shake flasks cultures. Obtained results indicated that yeast extract can be used to increase ginsenoside production in hairy root cultures of P. quinquefolium.
Highlights
Elicitation is one of the most effective strategies used for enhancing the production of secondary compounds in in vitro cultures
Purpose of this study was determine the influence of different in concentrations and the purpose study was to determine the influence of different concentrations of this varied exposure time of onthis the saponin content in transformed roots of American ginseng cultured in the elicitor and varied time on the saponin content in transformed roots of American ginseng shake flasks andexposure the nutrient sprinkle bioreactor
This study evaluated the effect of different concentrations of yeast extract on the production of ginsenosides in transformed root cultures of Panax quinquefolium in the shake flasks and the nutrient sprinkle bioreactor depending on the exposure time of elicitor
Summary
Elicitation is one of the most effective strategies used for enhancing the production of secondary compounds in in vitro cultures. This process consists of adding elicitors to the culture medium. Abiotic elicitors (AE) have no biological origin and they include physical (UV radiation, usage of freeze-thaw cycles, osmotic stress, salinity, drought) and chemical (mineral salts, heavy metals and chemical compounds capable of DNA and cell membrane damage: detergents, fungicides and herbicides) factors [2]. Biotic elicitors (BE) are substances of biological origin. They may be derived from a pathogen (exogenous elicitors) or from the plant (endogenous elicitors).
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