Abstract
Components of the filling valve in a gravity filling machine namely, tulip rubber, spreader rubber and vent tube were analyzed for yeasts using the membrane filtration method. After 5 days incubation, it was found that the tulip rubber had the highest yeast count of 9 cfu/20mls while the vent tube had the least count of 5 cfu/20mls. This indicates that the tulip rubber has the highest potential for contaminating finished products. Therefore, the tulip rubbers should be taken as a critical control point for control of yeast contamination during carbonated soft drink production. Journal of Applied Sciences and Environmental Management Vol. 10(3) 2006: 157-158
Highlights
It was left standing for 10 minutes after which the swab stick was discarded and the water filtered using a membrane filtration apparatus
Vacuum was applied to draw the sample through a 0.65 micron cellulose nitrate filter paper (Anon, 2002)
The filter paper was placed on Schaufus pottinger media (Satorius) prepared according to manufacturers instruction and incubated at 30o C for five days
Summary
Yeasts are useful in bakery and breweries but undesirable in carbonated soft drink industries due to their ability to ferment sugar (Nester et al 1983). Sugar fermentation causes the product to change color and taste which results in a shorter shelf life (Anon, 1997). If a carbonated soft drink manufacturer keeps the bottling environment yeast free, it shows that they are in control of the environment. It was left standing for 10 minutes after which the swab stick was discarded and the water filtered using a membrane filtration apparatus.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
