Yeast cell wall mannan rich fraction modulates bacterial cellular respiration potentiating antibiotic efficacy

Helen Smith,Richard Murphy,Sharon Grant,Joanne Parker

doi:10.1038/s41598-020-78855-5

Helen Smith, Richard Murphy + Show 2 more

Open Access

https://doi.org/10.1038/s41598-020-78855-5

Copy DOI

Journal: Scientific Reports	Publication Date: Dec 1, 2020
Citations: 18	License type: open-access

Affiliation: Alltech (Ireland)

Abstract

Now more than ever there is a demand to understand the mechanisms surrounding antibiotic resistance and look for alternative ways to impact phenotypic antibiotic outcome. Cellular energetics can be impacted by many bacteriostatic and bactericidal antibiotics, which affect metabolism and energy output, resulting in a reduction of cell growth or induction of cell death respectively. In this study, we provide evidence that a mannan rich fraction (MRF) from the cell wall of Saccharomyces cerevisiae modulates growth of antibiotic susceptible and resistant Escherichia coli and potentiates bactericidal antibiotic efficiency through modulation of bacterial cellular respiration. The role of MRF in modulating bactericidal impact and cellular metabolic state were assessed in E. coli by monitoring microbial growth and by measuring oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) using the Seahorse XFe96 Analyser, respectively. This work further illustrates the link between bacterial susceptibility to antibiotics (phenotypic resistance) and resistance through modulation of bacterial metabolism. This is the first example of yeast MRF enabling collateral sensitivity to antibiotics in vitro and supports the search for alternative strategies to promote animal health without contributing to the growing issue of antimicrobial resistance.

Highlights

More than ever there is a demand to understand the mechanisms surrounding antibiotic resistance and look for alternative ways to impact phenotypic antibiotic outcome
We examine if mannan rich fraction (MRF), a derivative of MOS, potentiates growth of antibiotic susceptible and resistant E. coli in conjunction with bactericidal antibiotic treatment
When MRF was supplemented (≥ 0.1% (w/v)) in the media of antibiotic susceptible E. coli, with no ampicillin treatment, a significant reduction in final growth measurement (p ≤ 0.01) and maximum optical density (OD) (p ≤ 0.05) was observed [Figs. 1a and 2a ( Table S1 online)]

Summary

Introduction

More than ever there is a demand to understand the mechanisms surrounding antibiotic resistance and look for alternative ways to impact phenotypic antibiotic outcome. We provide evidence that a mannan rich fraction (MRF) from the cell wall of Saccharomyces cerevisiae modulates growth of antibiotic susceptible and resistant Escherichia coli and potentiates bactericidal antibiotic efficiency through modulation of bacterial cellular respiration. This work further illustrates the link between bacterial susceptibility to antibiotics (phenotypic resistance) and resistance through modulation of bacterial metabolism This is the first example of yeast MRF enabling collateral sensitivity to antibiotics in vitro and supports the search for alternative strategies to promote animal health without contributing to the growing issue of antimicrobial resistance. This research is timely with respect to the search for alternate strategies to promote animal health without contributing to antimicrobial resistance

Methods

Results

Conclusion