Abstract
The transcriptional coactivator YAP1 is a critical effector of the human Salvador-Warts-Hippo pathway. Literature data report apparently discrepant results on the carcinogenic role of YAP1, which acts either as oncogene or as tumor suppressor in different in vitro and in vivo models. Furthermore, genomic amplification events of 11q22 locus encompassing YAP1 gene have been detected in multiple tumor types but there is limited direct evidence about the oncogenic role of endogenous YAP1 within in the amplicon. We screened a panel of human tumor samples and cancer cell lines and identified that the YAP1 amplification event is actually present in up to 23% of the cases. We exploited EKVX (lung cancer), CaSki (cervical cancer) and RO82 (thyroid cancer) cell lines harboring both genomic YAP1 amplification and YAP1 protein overexpression, in order to study the effects of downregulation of endogenous YAP1 by RNA-interference strategies. Class comparison analysis of gene expression profiling data identified 707 statistically significantly modulated genes (multivariable global test p-value = 0.002) that were functionally annotated for cell proliferation and cellular movement ontologies. Mechanistic studies of the identified perturbed pathways revealed that YAP1 silencing significantly decreased cell proliferation and cell cycle perturbation associated with upregulation of p21 and p27 cell-cycle inhibitors, reduced cell migration (p<0.048) and anchorage-independent growth (p<0.02). In CaSki cell line, YAP1 silencing induced significantly increased sensitivity and cell-death response to cisplatin treatment (p=0.011) as well as reduction of in-vivo tumorigenic potential (p=0.027). Overall, these results establish that YAP1 is a direct oncogenic target of the 11q22 amplicon in previously unreported cancer types and support the relevance of such genetic aberration in carcinogenesis in a fraction of multiple tumor types.
Highlights
The recently described Salvador-Warts-Hippo pathway (SWH or Hippo pathway) is highly conserved throughout evolution [1] and is crucially involved in organ size regulation [2,3]
Mechanistic studies of the identified perturbed pathways revealed that YAP1 silencing significantly decreased cell proliferation and cell cycle perturbation associated with upregulation of p21 and p27 cell-cycle inhibitors, reduced cell migration (p
We focused our attention on tumor subtypes with little or no established involvement of YAP1 gene, and selected representative cancer cell lines, including CaSki cell line (Cervical squamous cell carcinoma), RO82 cell line (Follicular thyroid carcinoma) and EKVX cell line (Non small-cell lung adenocarcinoma)
Summary
The recently described Salvador-Warts-Hippo pathway (SWH or Hippo pathway) is highly conserved throughout evolution [1] and is crucially involved in organ size regulation [2,3]. YAP1 point/small mutations have not been described so far and the reported 11q22 amplification events include multiple flanking genes in addition to YAP1. Some of these genes have been involved in cancer development, including a cluster of matrix metalloproteinase (MMP) genes [30], two members of the BIRC family of caspase inhibitors (BIRC2 and BIRC3) [31] and the progesterone receptor (PGR) [32]. We detect YAP1 copy number amplification in clinical series of different human tumor types and identify the downstream genes and pathways that are critical as YAP1 effectors in carcinogenesis
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