Yanang water extract exhibits a protective effect against methomyl-induced cytotoxicity in RAW 264.7 cells via suppression of apoptosis and cell cycle arrest

Abstract

Methomyl, an extremely hazardous substance, is widely used for controlling insects and pests in agricultural production. This compound can cause several illnesses in humans. In this research, we determined the alleviation potential of Yanang water extract (YWE) against methomyl-induced cytotoxicity in RAW 264.7 macrophage cells and examined the underlying mechanisms of action. The YWE (2.5 – 10 μg/mL) exhibited no toxicity against RAW 264.7 cells, whereas methomyl significantly reduced RAW 264.7 cell growth, resulting in the progression of apoptosis and cell cycle arrest. Supplementation of YWE on methomyl treated RAW 264.7 cells revealed a pronounced protective effect via the suppression of caspase-9 and caspase-3 mRNA expression levels. Proteomics studies were used to assess the effect of methomyl on RAW 264.7 cells, revealing an increase of proteins associated with apoptosis and cell cycle arrest, whereas in the YWE co-treatment condition these proteins were suppressed. Moreover, an increase in proteins involved with cell cycle progression and anti-apoptosis was found in YWE co-treatment. Our findings suggest that YWE is potentially involved in mitigating methomyl-induced cytotoxicity, via the suppression of apoptosis and cell cycle arrest.