Abstract
Yacon demonstrating prominent hypoglycemic action and having high content of biologically active compounds, is recognized for their ability to capture free radicals. This study is aimed to evaluate the influence of yacon leaves and root tubers water extracts and aqueous suspension of root tubers powder on rats' erythrocyte antioxidant defense system under the experimental diabetes mellitus type 1. Healthy animals and animals with diabetes mellitus were treated with water extracts of yacon leaves and root tubers at dose 70 or 500 mg / kg /day and with aqueous suspension of root tubers powder at dose 500 mg / kg /day or the same suspension which was stabilized by biocomplex PS. Thiobarbituric acid reactive substances (TBARS) and protein carbonyl content (PCC), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activity in erythrocytes lysates were measured. Water extracts of yacon leaves and root tubers and aqueous suspension of root tubers powder possess significant antioxidant effect, which was indicated by the decrease of TBARS and PCC and increase in SOD, CAT and GPx activity. Root tubers of yacon water extract at a dose of 500 mg/kg/day and aqueous suspension of root tubers powder stabilized by biocomplex PS possess he most pronounced antioxidant effect.
Highlights
Type 1 diabetes mellitus (DM) is a chronic T-cell– mediated autoimmune disease that results in the destruction of insulin-secreting β-cells
Experimental diabetes mellitus is accompanied by increase of thiobarbituric acid reactive substances (TBARS) by 164 % compared with erythrocytes lysates of the control group
Leaves and root tubers water extracts and aqueous suspensions of root tubers powder cause the reduction of TBARS in control animals respectively by 44 % (C + EL70), 47 % (C + EL500), 60 % (C + ER70), 37% (C + ER500), 56 % (C + S), 39 % (C + SPS) and in animals with diabetes by 75% (DM + EL70), 86 % (DM + EL500), 83 % (DM + ER70), 58 % (DM + ER500), 81 % (DM + S), 83 % (DM + SPS)
Summary
Type 1 diabetes mellitus (DM) is a chronic T-cell– mediated autoimmune disease that results in the destruction of insulin-secreting β-cells. Hyperglycemia is the most important factor of the onset and progress of diabetic complications. One of the most suitable models for the investigation of antioxidant defense system changes are red blood cells. Erythrocytes are continuously exposed to glucose in plasma during their circulatory life span. The glucose concentration in the erythrocyte cytosol is ensured by passive transport through GLUT1 (insulin-independent glucose transporter) and is close to that in the plasma and obviously increases under the conditions of hyperglycemia [5]. Red blood cells are susceptible to ROS attacks because of their high polyunsaturated fatty acid content and their abundance of iron (Fe2+)-rich hemoglobin and molecular oxygen. Ionic Fe2+ acts as a catalyst in redox reactions and lipid
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