Xylooligosaccharides benefits the growth, digestive functions and TOR signaling in Megalobrama amblycephala fed diets with fish meal replaced by rice protein concentrate

Abstract

This study was conducted to examine the effects of fish meal (FM) replacement by rice protein concentrate (RPC) supplemented with xylooligosaccharides (XOS) on the growth, intestinal digestive and absorptive capabilities and amino acid metabolism of blunt snout bream. Fish were randomly divided into six groups, and were fed with the control diet (containing 10% FM), the RPC diet (FM entirely replaced by RPC) and the RPC diet supplemented with 0.5, 1.5, 2.3 and 3% XOS, respectively, for 8 weeks. Fish fed the RPC diet obtained significantly lower values in daily growth index, feed efficiency ratio, nitrogen and energy retention and plasma concentrations of methionine, leucine, phenylalanine, lysine and histidine compared to the control. However, the supplement of 1.5% XOS restored these parameters to the levels similar to the control group. Fish fed the RPC diet showed no statistical difference in intestinal lipase, alkaline phosphatase and Na+K+-ATPase activities compared to the control group, but exhibited lower protease, carboxypeptidase A, aminopeptidase N and γ-glutamyl transpeptidase activities. However, these effects were reserved with the administration of 1.5% XOS. Also, the transmission electron microscopy analysis showed a significantly lower microvilli length in the mid-intestine of fish fed the RPC diet compared to the control, but it improved significantly with the supplementation of XOS. Hepatic activities of alanine transaminase, aspartate aminotransferase and glutamate dehydrogenase were significantly lower in fish fed the RPC diet compared to the control, but they improved with the supplementation of 1.5% XOS. Similarly, fish fed the RPC diet obtained relatively low expression levels of the target of rapamycin (TOR) and ribosomal protein S6 kinase 1 but higher expressions of eukaryotic translation initiation factor 4E-binding protein 2 in the intestine, liver and muscle. Opposite trend was observed in fish fed the RPC diet supplemented with 1.5% XOS. Overall, these results showed that RPC supplemented with 1.5% XOS could completely replace FM in the feed of blunt snout bream without any adverse effect on the growth performance, intestinal enzymes activities, gut morphology, amino acid metabolism and TOR signaling.