Abstract
Four xylanases of Cellulomonas flavigena were cloned, expressed in Escherichia coli and purified. Three enzymes (CFXyl1, CFXyl2, and CFXyl4) were from the GH10 family, while CFXyl3 was from the GH11 family. The enzymes possessed moderate temperature stability and a neutral pH optimum. The enzymes were more stable at alkaline pH values. CFXyl1 and CFXyl2 hydrolyzed xylan to form xylobiose, xylotriose, xylohexaose, xylopentaose, and xylose, which is typical for GH10. CFXyl3 (GH11) and CFXyl4 (GH10) formed the same xylooligosaccharides, but xylose was formed in small amounts. The xylanases made efficient saccharification of rye, wheat and oat, common components of animal feed, which indicates their high biotechnological potential.
Highlights
Hemicelluloses are polysaccharides which, along with cellulose and lignin, are the main polymer components of a plant cell wall
The main role in the destruction of xylan is played by xylanase, which catalyzes the random hydrolysis of xylan to xylooligosaccharides
Cultivation of microorganism, cloning, recombinant expression and purification of xylanases The strain C. flavigena Ac-1137 was obtained from the All-Russian Collection of Microorganisms (VKM, http:// vkm.ru)
Summary
Hemicelluloses are polysaccharides which, along with cellulose and lignin, are the main polymer components of a plant cell wall. Polysaccharide monomers, hemicelluloses are divided into xyloglucans, xylans, mannans, glucomannans, arabinoxylans, and β-glucans (Scheller and Ulvskov 2010). This polysaccharide consists of the β-(1-4) linked xylose backbone with a small amount of β-(1-3) branches, often with O-acetyl groups (Ebringerova and Heinze 2000). The main role in the destruction of xylan is played by xylanase (endo-1,4-β-xylanase, E.C. 3.2.1.8), which catalyzes the random hydrolysis of xylan to xylooligosaccharides. Another enzyme, β-xylosidase (xylan-1,4-β-xylosidase, E.C. 3.2.1.37), completes the
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