Abstract
Xylanase recovery using AOT-reversed micelles was evaluated under different experimental conditions. A full factorial design with centre point was employed to verify the influence of different factors on the recovery. A mathematical model represented xylanase yield (Y) as a function of pH and temperature: Y=8·75−4·31A2−3·13C2, where A=pH and C=temperature. The highest xylanase recovery, indicated by the model (10·2%), was attained at pH 6·3, AOT=0·57 M and temperature 46·8°C. A new xylanase extraction was performed under these conditions to test the model. The recovery yield obtained (9·1%) showed a close similarity between the experimental result and the value predicted by the model.
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