Xylanase, Laccase and Manganese Peroxidase Production from White Rot Fungi

Abstract

Xylanase, laccase and manganese peroxidase (MnP) were produced and optimized by three strains of white rot fungi Phanerochaetesordida MRL3, LentinuspigrinusMRL6 and PoliporuscaliatusMRL7. These strains were initially isolated from wood decaying samples and then screened on minimal salt media,xylanase activities for the above strains were 55, 77.4 and 64.8 IU/mL, respectively. In addition, laccase activities were 80.65, 112.91 and 101.61 U/L, respectively. The activities forMnP were 123, 182.6 and 106.6 U/L, respectively. The maximum xylanase activity was observed at pH: 5.0, 30°C after 216 hours of incubation period. The maximum activities were 272.7, 278.52 and 292.8 IU/mL and the total protein was 1.24, 1.2 and 1.16 mg/mL, respectively. The maximum laccase activity was observed after 192-216 hours of incubation period, at pH: 5.0 on 30°C, the activities were 483.9, 516.4 and 459.67 U/L and the total protein 1.03, 1.2 and 1 mg/ml, respectively. The MnP activities were observed after 192-216 hours, at pH: 5.0 on 30°C, the activities were 588.66, 645.16 and 585.27 U/L and the total protein 1, 1.1 and 0.96 mg/mL, respectively.