Abstract
Xuebijing injection is a complex herbal medicine, and clinical and experimental studies have shown that it has a significant effect on acute respiratory distress syndrome and multiple organ dysfunction syndrome. However, the majority of studies regarding Xuebijing injection have focused on serum inflammatory factors, and few studies have been carried out from the perspective of the protein and mRNA expression of inflammatory cytokines. In this study, 60 healthy rabbits of mixed gender were randomly assigned to a normal control group (CG), oleic acid group (model group; MG) and oleic acid + Xuebijing injection group (treatment group; TG). Rabbits of the CG were treated with normal saline through the ear vein, rabbits of the MG were injected with oleic acid (0.4 ml/kg) and rabbits of the TG received 0.4 ml/kg oleic acid + 10 ml/kg Xuebijing injection. Blood samples were collected from the common carotid artery of all rabbits of all groups 1 h after the ear vein was injected with the corresponding reagent, and was used to measure the arterial partial pressure of oxygen (PaO2) and of carbon dioxide (PaCO2). The activity of myeloperoxidase (MPO) was tested, and the protein and mRNA expression levels of interleukin (IL)-6 and IL-10 were determined. Rabbits of the MG exhibited evident respiratory dysfunction (PaO2 and PaCO2 were low), histopathological lung damage and overactive inflammatory responses (the expression of the proinflammatory cytokine IL-6 and the anti-inflammatory cytokine IL-10 was increased at the protein and mRNA levels). Following the administration of the Xuebijing injection, the inflammatory response of the rabbits was significantly reduced. Xuebijing injection raised PaO2 and PaCO2, weakened the activity of MPO in the lung tissue, downregulated the expression of the proinflammatory cytokine IL-6 and further increased the expression of the anti-inflammatory cytokine IL-10. These results demonstrated that Xuebijing injection improved the respiratory function of rabbits with acute oleic acid-induced lung injury by inhibiting IL-6 expression and promoting IL-10 expression.
Highlights
A previous epidemiological study has shown that acute lung injury (ALI) is common in clinical critical diseases
Xuebijing injection raised arterial partial pressure of oxygen (PaO2) and arterial partial pressure of carbon dioxide (PaCO2), weakened the activity of MPO in the lung tissue, downregulated the expression of the proinflammatory cytokine interleukin 6 (IL‐6) and further increased the expression of the anti‐inflammatory cytokine interleukin 10 (IL‐10). These results demonstrated that Xuebijing injection improved the respiratory function of rabbits with acute oleic acid‐induced lung injury by inhibiting IL‐6 expression and promoting IL‐10 expression
The objective of this study was to investigate the possible mechanisms by which Xuebijing injection exerts its effects in the treatment of ALI
Summary
A previous epidemiological study has shown that acute lung injury (ALI) is common in clinical critical diseases. Despite competent supportive therapy and care having been used, the mortality rate of ALI remains high and the effectiveness remains unsatisfactory [1]. ALI and its severe form, multiple organ dysfunction syndrome (MODS), remain a major public health problem. Understanding the pathogenesis of ALI/MODS and the search for novel methods to treat ALI/MODS has become currently urgent. This study focuses on the efficacy and the mechanisms of Xuebijing injection in the treatment of ALI/MODS. An early characteristic of ALI is the activation and recruitment of neutrophils in the lungs and the inflammatory effect induced by neutrophils [2].
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