Abstract
Objective To investigate the effect of Xuan Bi Tong Yu Fang (XBTYF) on angiogenesis via the vascular endothelial growth factor- (VEGF-) Notch1/delta-like 4 (Dll4) pathway. Materials and Methods. Sixty Sprague-Dawley rats were randomly divided into six groups: control, sham-operated, myocardial ischemia model, and XBTYF treatment at 3.2, 1.6, and 0.8 g/kg. Electrocardiography was performed to evaluate the successful establishment of the model. Hematoxylin-eosin staining and transmission electron microscopy were carried out to observe the morphology and mitochondrial structure in myocardial cells, respectively. TUNEL staining was performed to assess the degree of cell apoptosis. The expression of VEGF-A, Notch1, Dll4, Bcl2, Bax, caspase 3, caspase 9, and cytochrome-c (Cyt-c) was observed by western blot. Results XBTYF inhibited changes to the morphology and mitochondrial structure in cardiomyocyte and reduced cell apoptosis. Compared with the model group, XBTYF at all doses (3.2, 1.6, and 0.8 g/kg) reduced the expression of Notch1, Dll4, Bax, caspase 3, caspase 9, and Cyt-c, whereas expression of VEGF-A and Bcl2 was increased. Conclusion XBTYF attenuated mitochondrial damage and cell apoptosis while promoting the angiogenesis of cardiomyocyte. The associated mechanism may be related to the VEGF-Notch1/Dll4 pathway.
Highlights
Coronary heart disease (CHD) is characterized by myocardial ischemia, hypoxia, and necrosis. e “China Cardiovascular Disease Report 2017” [1] pointed out that cardiovascular diseases accounted for more than 40% of disease-related deaths among residents in China, which was much higher than the incidence of tumors and other diseases
Coronary artery bypass grafting is the main treatment for coronary artery disease, but as it is an invasive technique and incurs a high cost, its clinical application is affected. e main pathological feature of coronary heart disease is coronary artery stenosis, which leads to myocardial ischemia. erefore, therapeutic angiogenesis has gradually become highlighted in the recent 10 years. erapeutic angiogenesis promotes the release of proangiogenic factors from the ischemic myocardium through certain interventions, forming new small blood
When Xuan Bi Tong Yu Fang (XBTYF) was administered at the high doses (3.2 g/kg), only a small number of inflammatory cells were infiltrated and blood vessels proliferated slightly
Summary
Coronary heart disease (CHD) is characterized by myocardial ischemia, hypoxia, and necrosis. e “China Cardiovascular Disease Report 2017” [1] pointed out that cardiovascular diseases accounted for more than 40% of disease-related deaths among residents in China, which was much higher than the incidence of tumors and other diseases. Coronary heart disease (CHD) is characterized by myocardial ischemia, hypoxia, and necrosis. CHD has one of the highest mortality rates worldwide and is a high-risk condition among cardiovascular diseases. According to a report of the World Health Organization in 2011, China’s death toll from coronary heart disease ranks second in the world. E main pathological feature of coronary heart disease is coronary artery stenosis, which leads to myocardial ischemia. Erapeutic angiogenesis promotes the release of proangiogenic factors from the ischemic myocardium through certain interventions, forming new small blood. Krueger et al [2] found that 3D GF scaffolds significantly promote the growth and differentiation of C2C12 myoblasts. Shin et al [3] showed that 3D GOcoated PU foams strongly evoked spontaneous myogenic differentiation of C2C12 skeletal myoblasts without any myogenic factors. Probable long-term side effects, and biocompatibility [8], it is still not an ideal CHD treatment strategy
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
