X-ray evidence for the elongation of thin and thick filaments during isometric contraction of a molluscan smooth muscle.

Abstract

The elongation of thin and thick filaments during isometric contraction of a molluscan smooth muscle was studied by measuring spacing changes of meridional reflections in the medium-angle X-ray diffraction pattern. X-ray patterns from the anterior byssus retractor muscle of Mytilus edulis in the resting, active, and catch states were taken from the same part of a muscle bundle at a fixed specimen-to-detector distance, using imaging plates and 10 s exposure to synchrotron radiation. The third-order reflection (9.2 A) of the axial period of actin, and the fourteenth-order reflection (10.4 A) of the axial subunit-repeat of the thick filament are increased in spacing in the active and catch states. From accurately measured changes in the axial distance of the 9.2 A layer line from the origin, thin filament elongations in the active and catch states are estimated to be 0.48 and 0.32%, respectively, in a muscle that maintains a tension of 12.2 kg cm-2 in the active state and 9.8 kg cm-2 in the catch state. Thick filament elongations in the active and catch states are similarly estimated to be 0.33, and 0.28%, respectively, based on the axial shift of the 10.4 A reflection. The 0.48% elongation of the thin filament in the active state agrees with an elongation that is presumed by White and Thorson (1973) to estimate the lower limit of the thin-filament stiffness. It seems that in the catch state the activated and resting thin filament structures are intermixed. The activated parts of the thin filament are probably more elongated than the apparent value, 0.32%.