X-ray diffraction testing for weak-binding crossbridges in relaxed bony fish muscle fibres at low ionic strength

Abstract

Equatorial X-ray diffraction patterns from single skinned fibres from bony fish muscle (turbot) were obtained with the fibres at 6°C bathed in relaxing solutions of 170 down to 26 m M ionic strength. Diffraction patterns from rigor fibres were also obtained as controls. Unlike fibres from rabbit muscle, which show very clear evidence of substantial crossbridge formation at low ionic strength in what is mechanically a rapid equilibrium (“weakbinding”) state (Brenner et al. 1982), diffraction patterns from bony fish fibres showed only a small change in relative peak intensities at low ionic strength (26 m M) compared with normal (170 m M) ionic strength. However, there was a slight ordering of the filament lattice at low ionic strength. The specimen temperature used (about 6°C) was not far from the normal physiological temperature of the fish. Likewise, only a small change was seen by Xu et al. 1987 in patterns from frog fibres at low ionic strength at 2 to 6°C. (Rabbit fibres previously studied, where large changes were seen at temperatures of 5 to 20°C, were about 17 to 32°C below physiological.) The I 11 I 10 ratio for fish fibres at 26 m M ionic strength was actually lower than that for rabbit even at normal ionic strength. This may be associated with an intrinsic structural difference between these muscles or alternatively with the disordering of the crossbridge helix in rabbit muscle found at low temperature by Wray 1987, and could support the view that rabbit fibres at 5°C and normal ionic strength may already have a significant population of weak-binding crossbridges.