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Abstract
Ditylum Brightwelli, a marine plankton diatom, shows very peculiar osmotic relations which have recently been investigated by Gross (1939). The cells (fig. 1) consist of a central mass of protoplasm containing the nucleus and connected by a few protoplasmic strands with the peripheral plasma membrane in which are embedded numerous chromatophores. The remainder of the space inside the cell is filled with cell sap. As in other diatoms, the protoplast is enclosed by a siliceous cell wall. Gross found thatDitylumplasmolyses rapidly in isotonic and hypotonic NaCl and sugar solutions, being reduced in a few seconds to a small spherical body about one-third to one-twentieth of its original volume, similar in all respects to a resting spore. In a solution of NaCl + CaCl2, and in unbuffered artificial sea-water, plasmolysis occurs in all cells, but the process is considerably delayed and completed only after several hours. In artificial sea-water, the pH of which is adjusted to about 8, the cells remain unchanged.
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