XcmI-Containing Vector for Direct Cloning of PCR Products

Abstract

BioTechniquesVol. 22, No. 5 BenchmarksOpen AccessXcmI-Containing Vector for Direct Cloning of PCR ProductsAlex Y. Borovkov & Mark I. RivkinAlex Y. BorovkovNorth Dakota State University, Fargo, ND, USASearch for more papers by this author & Mark I. Rivkin*Address correspondence to Mark I. Rivkin, Department of Plant Sciences, Loftsgard Hall, North Dakota State University, P.O. Box 5051, Fargo, ND 58105-5051, USA. Internet: E-mail Address: rivkin@plains.nodak.eduNorth Dakota State University, Fargo, ND, USASearch for more papers by this authorPublished Online:7 Aug 2018https://doi.org/10.2144/97225bm04AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInRedditEmail "XcmI-Containing Vector for Direct Cloning of PCR Products." , 22(5), pp. 812–814FiguresReferencesRelatedDetailsCited ByCloning Polymerase Chain Reaction (PCR) Products: Making T Vectors1 June 2021 | Cold Spring Harbor Protocols, Vol. 2021, No. 6A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products23 April 2019 | Scientific Reports, Vol. 9, No. 1UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase is indispensable for oogenesis, oocyte-to-embryo transition, and larval development of the nematode Caenorhabditis elegans16 November 2018 | Glycobiology, Vol. 29, No. 2Genetic modification of the carotenoid pathway in the red yeast Xanthophyllomyces dendrorhous: Engineering of a high-yield zeaxanthin strainJournal of Biotechnology, Vol. 289Development of Xanthophyllomyces dendrorhous as a production system for the colorless carotene phytoeneJournal of Biotechnology, Vol. 247Combinatorial Biosynthesis of Novel Multi-Hydroxy Carotenoids in the Red Yeast Xanthophyllomyces dendrorhous22 February 2017 | Journal of Fungi, Vol. 3, No. 1Why Johnny can't clone: Common pitfalls and not so common solutionsIchiro Matsumura3 April 2018 | BioTechniques, Vol. 59, No. 3Construction of a directional T vector for cloning PCR products and expression in Escherichia coliPlasmid, Vol. 79Annotation and functional assignment of the genes for the C30 carotenoid pathways from the genomes of two bacteria: Bacillus indicus and Bacillus firmusMicrobiology, Vol. 161, No. 1The Transcription Factor Cux1 in Cerebellar Granule Cell Development and Medulloblastoma Pathogenesis6 August 2014 | The Cerebellum, Vol. 13, No. 6A Novel Binary T-Vector with the GFP Reporter Gene for Promoter Characterization8 September 2014 | PLoS ONE, Vol. 9, No. 9Genetic engineering of the complete carotenoid pathway towards enhanced astaxanthin formation in Xanthophyllomyces dendrorhous starting from a high-yield mutant17 November 2013 | Applied Microbiology and Biotechnology, Vol. 98, No. 1pPCV, a versatile vector for cloning PCR products5 September 2013 | SpringerPlus, Vol. 2, No. 1Contribution of two ζ-carotene desaturases to the poly-cis desaturation pathway in the cyanobacterium Nostoc PCC 712022 May 2013 | Archives of Microbiology, Vol. 195, No. 7Multiple ketolases involved in light regulation of canthaxanthin biosynthesis in Nostoc punctiforme PCC 7310230 January 2013 | Planta, Vol. 237, No. 5Catalytic properties and reaction mechanism of the CrtO carotenoid ketolase from the cyanobacterium Synechocystis sp. 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