Xác định thành phần loài Rickettsia trên chuột và một số ngoại ký sinh trùng ở khu vực đóng quân một số đơn vị quân đội miền Bắc

Abstract

Rickettsia, a gram-negative bacterium, and an obligate intracellular bacterium, can potentially cause fatal acute fever if not promptly detected and treated. It's often transmitted to humans by arthropods like ticks, fleas, and lice. Rodents and small mammals can serve as key hosts for various Rickettsia species. Our study aimed to identify Rickettsia species using nested PCR and gene sequencing of ompA, ompB, 17-kDa, and gltA genes based on 29 DNA samples, which tested positive for Rickettsia spp collected from mice, ticks, fleas, and lice at military units in Lang Son, Nghe An, Hai Duong, and Bac Giang, The phylogenetic analysis showed that 9/29 (31%) of Rickettsia samples belonged to Rickettsia felis species, 1/29 (3.5%) of Rickettsia samples exhibited a 99.5% ompB gene sequence similarity to Rickettsia heilongjiangensis species .