Xanthophyll-cycle dynamics and rapid induction of anthocyanin synthesis in Eucalyptus nitens seedlings transferred to photoinhibitory conditions

Abstract

The effects of rapid induction and subsequent relaxation of cold-induced photoinhibition on pigment chemistry of Eucalyptus nitens (Deane and Maiden) Maiden seedling leaves was assessed. The seedlings were subjected to four treatments in a nursery, fertilised or non-fertilised and shaded or non-shaded, before induction of photoinhibition in a growth chamber. Within 2 days, growth chamber conditions decreased photochemical efficiency ( F v / F m ) and increased xanthophyll-cycle conversion ratio. This was associated with decreased levels of total chlorophyll and increased levels of xanthophyll-cycle pigments. After 8 days, F v / F m of all treatments rose. Anthocyanin levels gradually increased until day eight, except in non-shaded, non-fertilised seedlings, which had high levels before the induction of photoinhibition. Visible spectroscopy indicated increased absorption between 500 and 590 nm, indicative of anthocyanin absorption. Electron transport rate after xanthophyll-cycle relaxation was half that measured before induction of photoinhibition. The findings indicate that anthocyanins may provide a photoprotective role in E. nitens seedlings under conditions of photoinhibition.