Abstract
In 1981, symptoms resembling those of citrus bacterial canker disease were observed on Mexican lime leaves and twigs in Colima, Mexico. Isolations from leaf pustules and dew drops on leaves occasionally included bacteria culturally resembling Xanthomonas spp. Five of six lesion extracts from the field reacted positively in indirect F(ab’)2-based enzyme-linked immunosorbent assays using antisera prepared against intact cells of two strains of X. campestris pv. citri. Five strains from Colima were identified as X. campestris on the basis of reactions to the following tests: Gram reaction; flagellum configuration; acid production from several carbon sources; utilization of L-asparagine; catalase reaction; starch hydrolysis; protein digestion; urease production; gelatin liquefaction; indole production; H2S production; aesculin hydrolysis; pigment properties; fatty acid analysis; and serological affinity with X. campestris pv. citri. Artificial leaf inoculations with these strains caused lesions similar, but not always identical, to those occurring in the field. Based on bacterial properties and pathogenicity on citrus, the Colima strains are provisionally identified as X. c. pv. citri.
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