X-ray structure of a galactose-specific lectin from Spatholobous parviflorous

Abstract

A galactose-specific seed lectin from Spatholobous parviflorus (SPL) has been purified, crystallized and its X-ray structure solved. It is the first lectin purified and crystallized from the genus Spatholobus (family: Fabaceae). The crystals belong to the space group P1, with a=60.792Å, b=60.998Å, c=78.179Å, α=78.68°, β=88.62°, γ=104.32°. The data were collected at 2.04Å resolution under cryocondition, on a MAR image-plate detector system, mounted on a rotating anode X-ray generator. The coordinates of Dolichos biflorus lectin (1lu1) were successfully used for the structure solution by molecular replacement method. The primary structure of the SPL was not known earlier and it was unambiguously visible in the electron density. S. parviflorus lectin is a hetero-dimeric-tetramer with two alpha and two beta chains of 251 and 239 residues respectively. SPL has two metal ions, Ca2+ and Mn2+, bound to a loop region of each chain. The SPL monomers are in jelly roll form.