Abstract
Interference fringes on the 14.5 nm meridional reflection (M3) are generated by diffraction from the arrays of myosin crossbridges in the two halves of each thick filament. The thick filaments are all constructed -in identical fashion (or nearly so), and all have H-zones of the same width, so that the interference distance between the two diffracting arrays in each filament is the same, and so is the pattern each gives. The separation between the fringes is inversely proportional to this interference distance (about 900 nm) so that a very high-resolution camera is necessary to resolve them. At the M3 reflection, one is seeing fringes of approximately the 62nd order, so that small changes in interference distance are magnified by this factor, and, for instance, a 1% change in distance will shift the position of the fringes by more than one half a fringe width.
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