Abstract
Sensitivity to X-ray-induced G 2 arrest was compared between ataxia telangiecstasia (AT) lymphoblastoid cells and normal human cells. Flow cytometrical analysis of cells following X-ray irradiation revealed that the fraction of cells with 4n DNA content was greater in T cells than in normal cells as previously reported by other investigators. However, the other parameters for cell-cycle progression kinetics including mitotic indices, cumulative mitotic indices and cumulative labelled mitotic indices indicated that X-ray-induced G 2 arrest as a function of dose in AT cells was indistunguishable from that in normal cells. Moreover, no significant difference in cell viability was notd between AT and normal cells until 48 h following X-irradiation up to 2.6 Gy, although X-irradiated AT cells, compared to normal cells, showed a significantly decreased survival in terms of cell multiplication in growth medium and colony formation in soft agar. These data collectively suggest that the greater accumulation of AT cells with 4n DNA content in flow cytometry cannot be attributed to mor stringent irreversible blockage of cell-cycle progression at the G 2 phase and eventual cell death there. The possible reasons fro this greater accumulation are discussed.
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