Abstract
Abstract Callus cultures of various cultivars of Vicia faba were studied for regeneration. During callus growth on agar or in liquid medium a black tissue was often observed correlated with an autolysis of some cells. With those tissues callus growth and regeneration of plants was inhibited. In black callus the phytoalexin synthesis is induced without infection by microorganism. The main phytoalexins in the callus tissue are wyerol and wyerone, with some wyeronic acid and dihydrowyerone. Wyerol was found especially in the cultivar Troy at a high concentration. In four cultivars the pooled phytoalexin concentration varied between 10 and 150 μg · g-1 fresh weight.
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